An integrated approach is presented that allows registering allelopathic manifestations of Quercus robur at different levels of the morphological organization of test objects. The results of the study of biological samples of allelochemicals Q. robur by the method of recording the delayed fluorescence of chlorophyll Chlorella vulgaris Beijer are discussed; by the method of biological samples A.M. Grodzinsky with watercress as a test object, as well as the results of an analysis of the heterogeneity of the vegetation cover of Quercus robur habitats from the standpoint of identifying ecological-cenotic groups. Regularities of the enhancement of the allelopathic effect with an increase in the concentration of solutions, a greater inhibitory effect of bioassays on test objects taken from natural communities of Quercus robur in comparison with similar samples from artificial plantings in Chelyabinsk were revealed.